Abstract
The current study was carried out, to explore the Antioxidant and antibacterial potential of the crude methanolic extract of selected species and estimation of antibacterial activities of the selected Artemisia species (A. absinthium L.). The antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assay, the methanolic extract displayed the highest scavenging activity in (DPPH) was (89.58 mu g/ml) on Artemisia absinthium L. root and the lowest was 36.14 mu g/ml on Artemisia absinthium L. leaves, while in (ABTS) the highest activity was 93.35 mu g/ml on Artemisia absinthium L. stem and lowest was 46.31 mu g/ml on Artemisia absinthium leaves. In minimum inhibitory concentration assay the crude methanolic extracts showed significant inhibition against all tested microbes on different concentrations like 25 mu g/ml, 50 mu g/ml, and 100 mu g/ml. The extract (Artemisia absinthium leaves) AaL showed MIC of 12.5 mu g/ml for B. subtilis which is gram positive bacteria, 12,5 mu g/ml for gram positive bacteria S. aureus and 6.25 mu g/ml for gram negative bacteria P. aeruginosa that is almost equal to the response of standard ciprofloxacin. Our current study revealed that Artemisia absinthium L. root (AsR) exhibited a significant antioxidant potential while AaL showed good antibacterial effect which is suggested to be used for treatment and management of different infectious diseases.