Abstract
From the Leaves of
Viburnum tinus L. four metabolites viz viburtinoside A and B (acylated iridoid glucosides), scopoletin 7-
O-β-
d-sophoroside and natural occurred 2,6-di-
C-methyl-nicotinic acid 3,5-diethyl ester with 10 known metabolites were isolated. Toxicity and CCl
4-induced hepatotoxicity of the investigated extract have been evaluated in terms of the determination of ALT, AST, lipid peroxide and nitric oxide levels in serum.
From the leaves of
Viburnum tinus L. (Adoxaceae) two acylated iridoid glucosides (viburtinoside A and B), a coumarin diglucoside scopoletin 7-
O-β-
d-sophoroside and a natural occurred dinicotinic acid ester 2,6-di-
C-methyl-nicotinic acid 3,5-diethyl ester were isolated. In addition to these, 10 known compounds were isolated, namely two bidesmosidic saponins, a hexamethoxy-flavone and five flavonol glycosides, as well as suspensolide A and oleanolic acid were isolated for the first time in this genus and species, respectively. The structures were determined mainly by spectroscopic methods (UV, IR, ESI-MS,
1H-,
13C NMR and DEPT). Toxicity of the investigated extract was determined (LD
50
=
500
mg/kg). CCl
4-induced hepatotoxicity has been evaluated in terms of the determination of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lipid peroxide and nitric oxide levels in serum and compared using adult male rats weighing 150–180
g. Their highly elevated levels were significantly reduced by treatment with the investigated aqueous methanol extract in dose-dependant manner.