Abstract
The study was carried out to assess the phytochemical and antimicrobial bioassay of five medicinal plants, Lepidium sativum, Nerium oleander, Ranunculus repens, Tecoma stans and Urtica dioca. These plants are traditionally used as medicine in the Northwest Pakistan, therefore it is necessary to identify and estimate their alkaloid, flavonoid, saponin, phenol and tannin contents. Phytochemical inveigation of plant samples determines that alkaloid (63.6%) and flavonoid (0.91%) were highest in N. oleander, saponin (11%) and phenol (0.031) in T. stans, tannin (0.61%) in L. sativum, All five species showed no significant antimicrobial activities.