Abstract
EXPLORATION of novel and effective therapies against liver damage with antioxidant activity is a point worth studying. The present study was aimed to evaluate the hepatoprotective and antioxidant activities of Euphorbia cooperi N. E. Br. aerial parts in paracetamol-induced hepatotoxicity mice and to isolate the bioactive constituents. Our findings suggest potential antioxidant and hepatoprotective activity of the n-butanol fraction of the plant, as it significantly (P<0.05) prevented the increased serum marker enzymes aspartate aminotransferase andalanine aminotransferase and bilirubin levels, decreased hepatic malondialdehyde, and recovered glutathione levels. Chromatographic separation of n-butanol fraction led to isolation of seven compounds, namely, gallic acid, brevifolin carboxylic acid, kaempferol-3-O-ss-D-rutinoside, corilagin, 3, 3'-dimethoxy ellagic acid, 3, 4, 4'-trimethoxy ellagic acid, and ellagic acid. The structure of isolated compounds was elucidated by physical and spectroscopic analysis and by comparison with the literature data. These compounds are isolated for the first time from E. cooperi N. E. Br.