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Pilot-Scale Production of Fatty Acid Ethyl Esters by an Engineered Escherichia coli Strain Harboring the p(Microdiesel) Plasmid
Journal article   Peer reviewed

Pilot-Scale Production of Fatty Acid Ethyl Esters by an Engineered Escherichia coli Strain Harboring the p(Microdiesel) Plasmid

Yasser Elbahloul and Alexander Steinbuechel
Applied and environmental microbiology, Vol.76(13), pp.4560-4565
01/07/2010
DOI: https://doi.org/10.1128/AEM.00515-10
PMCID: PMC2897437
PMID: 20453138

Abstract

Biotechnology & Applied Microbiology Life Sciences & Biomedicine Microbiology Science & Technology
Fatty acid ethyl esters (FAEEs) were produced in this study by the use of an engineered Escherichia coli p(Microdiesel) strain. Four fed-batch pilot scale cultivations were carried out by first using glycerol as sole carbon source for biomass production before glucose and oleic acid were added as carbon sources. Cultivations yielded a cell density of up to 61 +/- 3.1 g of cell dry mass (CDM) per liter and a maximal FAEE content of 25.4% +/- 1.1% (wt/wt) of CDM.

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