Abstract
In the present study, polyacrylamide gel (PAG) was utilized as bolster material for the immobilization of in-house extracted and partially purified manganese peroxidase (MnP) through entrapment technique. The entrapment technique impelled incredibly compelling MnP immobilization (87.3±3.3%) and conferred remarkable stability to the enzyme (37.2±2.4%) following two months of storage at 4°C. The PAG-assisted immobilization expanded the reaction time of MnP after 10 min of response when contrasted with a partially purified free MnP counterpart, which demonstrated the highest activity after 5.0 min. Following PAG-assisted immobilization, an improvement in the optimal temperature and a chemical (an alkaline) shift in the pH optima of MnP were recorded. Moreover, a significant enhancement in the thermo-stability was also observed after immobilization. After 72 h, PAG-entrapped-MnP exhibited 41.2% residual activity at 50°C, whereas the free counterpart lost its activity completely under the same conditions. Furthermore, the PAG-entrapped-MnP also showed an excellent recycling efficiency and retained more than 50% of its initial activity after five consecutive reaction cycles. In conclusion, owing to the economic feasibility, carrier-supported MnP may be a promising candidate for various applications in different sectors of the modern world.