Abstract
Electrochemical, structural and morphological studies were performed on polyaniline film prepared in different solvents and electrolytes. For this purpose, cyclic voltammetry, fourier transformed infrared spectroscopy, scanning electron microscopy and atomic force microscopy were successively applied. Based on neutravidin entrapment inside the electrogenerated polyaniline followed by specific Fab fragment-antibody grafting, atrazine immunosensor was developed. The impedance measurements with polyaniline polymer reveal that immunosensor could detect significantly 0.01 ng/ml atrazine. This lower limit detection is due to the delocalized electron structure present in the polyaniline film. For unspecific detection, no response was recorded after injection of different concentrations of rabbit IgG.