Abstract
Synergistic strategy is always supported by nature due to multi-targets synergistic mode. Spices are one of the most nutritional products present in our diet, and dietary manipulation has been implicated in managing various medical conditions. Our interest was to study the effect of polyherbal formulation (PHF), composed of fenugreek, black seed, cumin, and flaxseed, in the experimental model of diabetic nephropathy (DN). After induction of diabetes (streptozotocin 55 mg/kg) in male Wistar rats, PHF (200 mg/kg) was given for 56 days. Biochemical and histopathological indicators were used to study nephritis. The diabetic group had elevated glucose, dyslipidemia, and impaired renal function. PHF treatment improved glycemic control, renal function, and dyslipidemia. Consecutively, oxidative damage marked as the elevated level of 8-hydroxy-20-deoxyguanosine (8-OHdG), transforming growth factor-beta1 (TGF-beta 1) and malonaldehyde (MDA) with concomitantly decreased glutathione (GSH), glutathione reductase (GR) and superoxide dismutase (SOD) depicted in the diabetic group were restored after the administration of PHF. Further histological analysis was done and explored as glomerulosclerosis and interstitial fibrosis in the diabetic group. Administration of PHF significantly modulated renal histological variations in diabetic rats. A current investigation concluded that PHF exhibited a strong nephroprotective effect by preserving renal damage due to its antidiabetic and antioxidant actions. Thus, PHF may find a pharmaceutical candidature to manage diabetes-related complications requiring more studies. (C) 2022 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).