Abstract
The present investigation was carried out to examine the presence of Booroola FecB mutation gene in 124 selected prolific ewes from 6 flocks representing two Saudi Arabian local sheep breeds by forced PCR-RFLP method. Genomic DNA was extracted from the blood of 69 Najdi and 55 Naeimi matured ewes with litter size varied from 2.01-2.16 lambs per ewe lambing. Digestion of FecB gene 190 base pair with AvaII restriction enzyme resulted in non carrier 190 bp band wild type in all samples which revealed the absence of this mutation in the tested Najdi and Naeimi sheep breeds.