Abstract
Polysaccharides are structurally complex and essential constituents of life, and therefore, studies directed to these kinds of molecules have received scientific attention. Despite an easy availability of
Dolichos biflorus
Linn and
Trachyspermum ammi
(Linn) seeds isolation, characterization and antimicrobial studies of polysaccharides derived from these two natural sources have not been investigated. Therefore, we report here isolation of polysaccharides, their purification and characterization from
Dolichos biflorus
Linn and
Trachyspermum ammi
(Linn) seeds. Gel permeation chromatography, GC–MS, SEM, XRD, EDX and FT-IR analyses show the presence of three pentose sugar such as
d
-ribose,
d
-arabinose,
d
-xylose and hexose sugar such as
d
-mannose,
d
-galactose and
d
-glucose. Unprecedented antimicrobial activity of these polysaccharides against Gram positive bacteria such as
Staphylococcus aureus
and
Bacillus subtilis
and Gram negative bacteria such as
Escherichia coli
and
Pseudomonas aeruginosa
are established.