Abstract
The genetic diversity and differentiation of four geographic populations of Neoschongastia gallinarum were evaluated using concatenated mitochondrial gene sequences (pCOI, pCOII, and pND5). Based on the results, the N. gallinarum populations had high genetic diversity and strong ecological adaptability. Genetic differentiation among paired populations calculated using concatenated mitochondrial gene sequences revealed that geographic isolation resulted in genetic differentiation among the populations of N. gallinarum, and gene flow between populations associated with human trade activities. Systematic development and molecular variance based on haplotypes revealed that genetic variation existed in different haplotypes; however, no clear rule related to geographic region was found. Further, genetic variation was mainly derived from individuals within the population. A neutral test based on concatenated mitochondrial gene sequences and nucleotide pair differences revealed that N. gallinarum did not experience an obvious population expansion in recent historical periods. Accordingly, the population size was relatively stable.