Abstract
Background and Objective: Monosodium glutamate (MSG) is an ordinarily utilized nourishment added substance and there is developing worry that this may play a critical role in the spermatogenesis dysfunction and may be lead to male infertility. The study was completed to explore the conceivable defensive impact of grape seeds extract (GSE) on testis toxicity induced by the flavor promoters, monosodium glutamate (MSG) in male rats. Materials and Methods: The investigation proceeded for about two months and the animal comprised of 80 male Wistar rats which were appropriated similarly among four groups. Testis weight, spermatozoa count, viability, live spermatozoa (%), sperm abnormalities (%), sex hormones, anti-oxidants enzymes, oxidative stress marker and testis histopathological investigations were recorded. All data was analyzed by one-way (ANOVA) at p<0.05. Results: The MSG treated rats caused significant diminish in testes weights and spermatozoa numbers, testosterone and Luteinizing (LH) but improvements in animals co-treated with GSE where significant incremented was reported. A significant (p<0.05) increment in plasma anti-oxidants (CAT and SOD) activities and a decrease in MDA level were observed with MSG+GSE compared with that MSG alone. Several changes were shown in the testis with epididymis in histological investigations. The testes seen indicated the decline of spermatogenic cells and distorted sertoli cells. The cell nuclei were seen pyknotic in leydig cells and the interstitial tissue showed up with various vacuoles and hemorrhage. Co-administration of GSE to MSG-treated rats improved the histopathological alterations induced by MSG in testis and increased the spermatozoa numbers. Conclusion: The results have clearly shown that GSE performs a very important defensive role during spermatogenesis and has the ability to act as a chemo-suppressive factor against MSG effects.