Abstract
A method for isolation of homogenous culture of cells expressing CD146 marker from adipose tissue lipoaspirate was developed. The resultant clonogenic cultures retained high proliferative activity, immunophenotype, and morphology after numerous passages. The presence of insulin in the medium served as the selective factor for maintenance of the population phenotype. These cultures effectively differentiate into CD31(+) endothelial cells and can be used in regenerative medicine.