Abstract
Hydroxyapatite was isolated from the chicken bones of Black Sumatra and Fighter cock by thermal calcinations at 700 °C. The reducing power of Plumbago indica was responsible for the green synthesis of silver NPs. The isolated hydroxyapatite was used for the preparation of hydroxyapatite doped silver nanoparticles (HAp/AgNPs) against both Gram-positive and Gram-negative bacteria. The Black Sumatra bone derived HAp/AgNPs were excellent activity against Klebsiella pneumoniae (28 mm), Staphylococcus aureus (26 mm), Bacillus cereus (24 mm), respectively. Fighting cock bones derived HAp/AgNPs demonstrated activity against Staphylococcus aureus (22 mm), Klebsiella pneumoniae (25 mm) and Bacillus cereus (23 mm), respectively. The MIC value ranged between 45 ± 1.2 µg/mL and 104 ± 0.25 µg/mL. The HAp/AgNPs were characterized by X-ray diffraction (XRD), Fourier Transformed Infrared spectroscopy (FTIR), and Scanning Electron Microscopy (SEM). The XRD patterns of the Hap/Ag revealed functional peaks representing the reflection planes. The HAp/AgNPs showed an ellipsoidal morphology in SEM analysis. The morphological studies at various magnifications indicated that HAp/AgNPs with good crystal structure could prepared by using co-precipitation method.