Abstract
The synthesis of pectin-mediated HAP Nanoparticle (pHAP) and Hydroxyapatite/Pectin Nanocomposite (HAP/P Composite) utilizing carrot pomace pectin as a template to support greener technologies was the focus of this research. To produce a regulated crystallinity, pectin is extracted from carrot pomace. Spectral techniques such as Fourier transform infrared spectroscopy (FTIR) to find out the chemical behavior of the compound, proton-1 nuclear resonance spectroscopy (1H NMR), and carbon-13 nuclear magnetic resonance spectroscopy (13C NMR) to identify the H and C atoms that were in the extricated pectin. FTIR, SEM, and XRD were used to assess the crystallinity, shape, and purity of the HAP nanoparticles. A protein denaturation assay and a proteinase inhibition assay were used to assess the anti-inflammatory effect of pectin-mediated HAP nanoparticles and Hydroxyapatite/Pectin Nanocomposite. According to the results, the pectin-mediated HAP nanoparticles generated in the presence of an optimal pectin concentration are pure, spherical, low crystalline, and discrete particles with a reduced size. Furthermore, it has antimicrobial properties. According to this study, the HAP nanoparticles incorporated utilizing pectin as a green template can operate as a useful biomaterial for biomedical purposes. MTT Assay and ALP Activity were performed on pHAP and HAP/Pectin Nanocomposites. When compared to pectin-mediated hydroxyapatite, the experimental results demonstrated that HAP/Pectin Nanocomposites have higher cell survival and bone mineralization. As a result, the HAP/Pectin Nanocomposites could be useful in biomedical applications.