Abstract
We are herein focusing on establishing an protocol for in vitro preservation of grapevine (Vitis vinifera) cv. Al-Bayadi. This was followed by determination of the genetic stability among six and twelve months post preservation by using three different techniques based on the DNA [Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR), inter simple sequence repeats (ISSR) and protein, Sodium dodecyl sulphate-polyacylamide gel electrophoresis (SDS-PAGE)]. Effect of carbon sources (Sucrose and Sorbitol) on preservation among six and twelve months as a slow growth technique was also determined. It was interesting that all DNA bands were of the monomorphic type which did not confirm the presence of the polymorphic and unique types of fragments, reflecting the inability of this technique to genetically differentiate between the treatments (genetic stability of the germplasm). On RAPD-PCR analyses, it was found that three types of fragments, i.e., monomorphic, polymorphic and unique fragments were recorded, which confirmed the genetic variability between the most and lowest efficient treatments. In case of ISSR technique, It was interesting that all DNA fragments were of the monomorphic type which did not confirm the presence of the polymorphic and unique types of fragments, reflecting the inability of this technique to genetically differentiate between the treatments. It was clear that protein analysis has shown some slight differences in treatments compared to control.As a conclusion, the use of RAPD-PCR and ISSR techniques post in vitro-preserved grapevine explants.