Abstract
A total of 280 Staph. aureus strains from a total of 1250 milk samples from buffaloes were tested for 15 antibiotics using disc diffusion method followed by detection of their respective antimicrobial resistant genes through PCR. Among them, the highest prevalence of Staph. aureus was found in Peshawar-Mardan division (30%), followed by Malakand (28.5%), Bannu-Dera Ismail khan division (25%) and Hazara division (16%). Over all the high resistance was found against Lin (96.25%) followed by AMX (82.5%), TET (63.75%), AMP (58.75%), SXT (50%), CHL (48.7%), CLR (36.25%), STR (25%), GEN (17.5%), OFX (15%), LFX (12.5%), AZM (8.75%) while least resistance against GAT (3.375%) and CRO (6.25%). Over all the highest prevalent gene was blaTEM (179) followed by tetA (147), tetB (144), blaCMY-2 (142), sul1 (139), sul3 (137), tetC (130), aadA (121), sul2 (118), strA/strB (117) while the least resistant gene was aaddB (12) and aac(3)IV (16).