Abstract
Total of 56 bacterial isolates were isolated from five palm tree cultivated soil samples and screened their amylase production. In the preliminary screening, twenty four isolates exhibited amylase producing ability. Among these 24 isolates, an isolate KSU-6 showed maximum amylase enzyme production (37.52 U mL(-1)), and the isolate was identified as Bacillus licheniformis KSU-6. The maximum amylase production was optimized, and the conditions were pH 10.0, temperature 50 degrees C, incubation period 44 h, inoculum concentration 2% (v/v), starch 0.2% (w/v) as carbon source, peptone 0.5% (w/v) along with malt extract 0.3% (w/v) as nitrogen sources for B. licheniformis KSU-6. The partially purified amylase stabled upto pH 12, temperature 80 degrees C, 1-2 M salt concentration and 10 mM of different detergents. In this study conclude that the soil bacterium Bacillus licheniformis KSU-6 produced thermostable and alkaline amylase, which could be useful for industrial liquefaction and detergent industries. (C) 2016 Friends Science Publishers