Abstract
Amylase producing actinobacteria were isolated and characterized from terrestrial environment. There are a limited number of reports investigating the marine environment; hence, in the present study, four marine enzymes were tested for their amylase production ability. On starch agar plates, the
strain showed a higher hydrolytic zone (24 mm) than the other isolates. Growth under optimized culture conditions using Plackett-Burman's experimental design led to a 1.7, 9.8, 7.7, and 3.12-fold increase for the isolates
,
,
, and
sp., respectively, in the specific activity measurement. When applying the Box-Behnken design on
using the most significant parameters (starch, K
HPO
, pH, and temperature), there was a 12.22-fold increase in the specific activity measurement 7.37 U/mg. The
-amylase was partially purified, and its molecular weight was determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
-Amylase was particularly active at pH 6 and 65°C. The purified enzyme was most active at 65°C and pH 6, thermal stability of 70°C for 40 min, and salt concentration of 1 M with Km and Vmax of 6.58 mg/ml and 21.93
mol/ml/min, respectively. The
-amylase was improved by adding Cu
, Zn
, and Fe
(152.21%, 207.24%, and 111.89%). Increased production of
-amylase enzyme by
KR108310 leads to production of significant industrial products.