Abstract
Porous silica monolith particles have been successfully prepared in an enhanced scale (80-85 g/batch) and derivatized with some ligands and packed in micro-columns. The separation efficiencies as high as 127 000 and 175 000 plates/m were achieved with 30 and 15 cm columns packed with C18 modified particles, respectively. The average particle size and particle size distribution were reduced by scale-up and subtle change of the formulation of reaction mixture. This phase also has shown some encouraging possibility for fast analysis. Another phase derivatized with mixed ligands of C8 and urea functionalities showed promising feasibility for proteomic analysis.