Abstract
As a kind of convenient, fast and the online analysis technology, point-of-care testing (POCT) technology has been widely used in disease diagnosis, health management, environmental monitoring and emergency response analysis. To date, it is a hot spot to develop novel strategy for the detection of various targets based on the simple, rapid and sensitive signal readout and different signal production, transfer and amplification strategies in the field of POCT. The introduction of some nanozymes and functionalized nanomaterials with special catalytic properties further accelerates the application of simple analysis signal readout such as color, temperature, pressure, weight and distance, which greatly promotes the development of simple readout-based POCT methods, and expands the analytical application of such methods. In this paper, we summarize the principles, classifications and the applications of these emerging POCT methods for the analysis of life-related targets and review their current challenges and fut