Abstract
A new nano optical sensor binuclear Pd-(2-aminothiazole) (urea), Pd(atz,ur) complex was prepared and characterized for the assessment of the activity of alpha amylase enzyme in urine and serum samples for early diagnosis of Pancreatitis disease. The assessment of alpha amylase activity is carried out by the quenching of the luminescence intensity of the nano optical sensor binuclear Pd(atz,ur) complex at 457nm by the 2-chloro-4-nitrophenol (2-CNP) which produced from the reaction of the enzyme with 2-chloro-4-nitrophenyl-α-d-maltotrioside (CNPG3) substrate. The remarkable quenching of the luminescence intensity at 457nm of nano Pd(atz,ur) doped in sol-gel matrix by various concentrations of the 2-CNP was successfully used as an optical sensor for the assessment of α-amylase activity. The calibration plot was achieved over the concentration range 8.5×10−6 to 1.9×10−9molL−1 2-CNP with a correlation coefficient of (0.999) and a detection limit of (7.4×10−10molL−1). The method was used satisfactorily for the assessment of the α-amylase activity over activity range (3–321U/L) in different urine and serum samples of pancreatitis patients. The assessment of the alpha amylase biomarker by the proposed method increases its sensitivity (96.88%) and specificity (94.41%) for early diagnosis of pancreatitis diseases.
•Nano optical sensor binuclear Pd(atz,ur) was prepared and characterized.•The optical sensor is used for early diagnosis of Pancreatitis diseases.•The diagnosis process depends on the assessment of alpha amylase in urine and serum samples.•The method was validated against the standard method.