Abstract
This study aimed to investigate the role of spirulina against Monosodium glutamate (MSG) induced hepatic dysfunction as an in vivo study in a rat model.
In this study, 28 rats were divided into four groups (i) controls, (ii) spirulina, (iii) MSG and (MSG + spirulina). Each group consists of 7 rats. The first group is considered as a normal group and consists of the availability of food and water without any restrictions. The second group involves 1000 mg/kg bw spirulina extract daily for ten days. The third group is the positive intoxicated group, given orally 4 g/kg bw monosodium glutamate (MSG) for ten days. The final group is orally administered Spirulina and MSG for ten days. Within each of the four groups, serum, qPCR, histology, and immunohistochemistry were examined.
Biochemical and antioxidant analysis showed a significant association with MSG and MSG + spirulina, and molecular analysis revealed a significant association with Nrf2, HO-1, BCl2, and β-actin genes. Histological analysis showed modest congestion of the central vein, intact hepatocytes organized in cords, and mild sinusoidal dilatation in the Centro lobular area of the liver of the spirulina-treated group. The H&E stain was documented to be 50–200 m bar. The liver section analysis in NF-κB, TGF-β and COX-2 showed a protective effect.
This study concludes as the combination of MSG and spirulina has a protective effect. However, MSG has shown an elevated role among all groups.