Abstract
One of the most prevalent harmful heavy metals is lead (Pb). It is generally recognized to be harmful to the testicles. Asparagus officinalis has many saponins, flavonoids, and other phenolics with strong antioxidant and anti-inflammatory effects. The effects of A. officinalis (asparagus) aqueous extract (AOAE) on testicular damage caused by lead acetate (PbAc) were investigated in this study. In this way, 20 mg/kg PbAc was injected intraperitoneally 2 h after mice were administered 400 mg/kg AOAE orally for 14 days. In the biochemical analysis of testicular tissue, PbAc decreased enzymatic and nonenzymatic antioxidant molecules in testicular tissue, while increasing lipid peroxidation, nitric oxide, inflammatory markers [nuclear factor kappa-B (NF-κB), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1 β), IL-6, and inducible nitric oxide synthase (iNOS)], and apoptotic-related proteins. Additionally, PbAc was discovered to reduce sperm motility and increase the percentage of dead sperm. However, due to its antioxidant qualities, AOAE has been found to reduce oxidative stress, therefore protecting against inflammation and apoptosis. It also allowed the AOAE sperm parameters to restore to their previous values in the control group. According to the findings, AOAE could be a natural substance that could be used to treat Pb-induced testicular toxicity; this protection may be attributed to its anti-oxidative, anti-inflammatory, and anti-apoptotic effects. However, this study warrants further works to explore in detail the underlying mechanisms of the alleviating effects of AOAE against Pb-induced toxicity and which of its active ingredients is responsible for this protection.