Abstract
Purpose:
To compare the ability of two active ingredients – sodium fluoride (NaF) and stannous fluoride (SnF
2
) – to inhibit hydroxyapatite (HAP) dissolution in buffered acidic media.
Methods:
Two
in vitro
studies were conducted. HAP powder, which is representative of tooth mineral, was pretreated with: test solutions of NaF or SnF
2
, 10 g solution per 300 mg HAP powder (Study 1); or NaF or SnF
2
dentifrice slurry supernatants, 20 g supernate per 200 mg HAP powder for 1 minute followed by three washes with water, then dried (Study 2). About 50 mg of pretreated HAP was exposed to 25 ml of acid dissolution media adjusted to and maintained at pH 4.5 in a Metrohn Titrino reaction cell. Exposure of HAP to the media results in dissolution and release of hydroxide ion, increasing the pH of the solution. The increase in pH is compensated for by automatic additions of acid to maintain the original pH (4.5) of the reaction cell. Total volume of titrant added after 30 minutes was used to calculate the percentage reduction in dissolution
versus
non-treated HAP control.
Results:
Both F sources provided protection against acid dissolution; however, in each study, SnF
2
-treated HAP was significantly more acid-resistant than the NaF treated mineral. In study 1, at 280 ppm F, representing concentrations of F found in the mouth after
in vivo
dentifrice use, the reduction in HAP dissolution was 47.7% for NaF and 75.7% for the SnF
2
-treated apatite (extrapolated). In study 2, the reduction in HAP dissolution was 61.3% for NaF and 92.8% for SnF
2
-treated samples. Differences in percentage reduction were statistically significant (Paired-
t
test).
Conclusions:
Results of these studies demonstrate that both of the fluoride sources tested enhance the acid resistance of tooth mineral and that resistance is significantly greater after treatment with SnF
2
compared with treatment of tooth mineral with NaF.