Abstract
Background: Despite the confirmed association between infant meningitis and necrotizing enterocolitis and C. sakazakii lettile known about the pathogenic interaction of C. sakazakii with host cells.
Aim: To characterize functional and regulatory proteins that are differentially expressed in Human brain microvascular endothelial cells (HBMEC) in response to meningitic C. Sakazakii
Method: Liquid Chromatography with tandem Mass Spectrometry (LC-MS/MS) analysis was performed to the protein extracted from HBMEC in response to meningitic C. Sakazakii in serial time course. Bioinformatics analysis was carried out using SCIEX OneOmics cloud processing software and Advaita's iPathway.
Results: In response to the exposure, the LC-MS/ MS analysis indicated different changes in 57 proteins: nucleic acid binding proteins, transporter proteins and structural molecule activity proteins. 1, 9 and 29 pathways were significantly affected after 60, 90 and 120 minutes, respectively. In addition, 179, 242 and 490 gene ontology components were found to be significantly enriched. Interestingly, the tight junction pathway, which is most important component regulating to brain damage, was triggered at 120 minutes post-infection with the C. sakazakii meningitic strain.
Conclusion: We conclude that the data presented in this study may facilitate a better understanding of this bacterium, which can cause irreparable damage to a new-born baby's brain. This study open up many new avenues into the research concerning this pathogenicity.