Abstract
A viral-specific polypeptide of molecular weight about 70,000 which does not incorporate radioactive glucosamine or fucose and which does not stain positively for carbohydrates is present in preparations of purified Rauscher murine leukemia virus. This polypeptide can be resolved from the major glycoprotein(s) previously designated
gp69
71
by high resolution sodium dodecyl sulfate-polyacrylamide-gel electrophoresis. The 70,000-dalton polypeptide is present in Rauscher virus-infected cells but not in nonproducer, uninfected N.I.H. Swiss mouse embryo cells. The [
35S]methionine-labeled tryptic peptide patterns of the virion and the intracellular 70,000-dalton polypeptide are identical, and each contains [
35S]methionine-labeled tryptic peptides characteristic of virion p30, indicating a possible precursor relationship to p30.