Abstract
Middle East respiratory syndrome coronavirus is the a genus belonging to beta corona Virus, currently well known for its out break in the countries like Saudi Arabia, Malaysia, Jordan, Qatar, Egypt, the UAE etc causing severe illness. MERS CoV is a dreadful RNA virus infecting huge population especially from Saudi Arabia. There is not been any protection against this well known virus in spite of its outbreak and rapid spread. The current work is an approach to design a vaccine that can protect the people by making them immune to the virus. The important proteins of the beta corona virus were retrieved from NCBI public data base and the screening was performed to identify the Foreign proteins among the complete set of viral proteins. The methodology is based on the principle that the diseases are caused due to the viral proteins that are totally foreign to the human proteome so that the synthesis of antibodies does not occur. The tools like BLAST of NCBI and TFASTY of SDSC Biology Workbench have been used for this screening. Once the foreign proteins were collected they were further analyzed for the presence of the antigenic peptide on their sequences. The tool used for the purpose was Antigenic Emboss. The result contains several peptides for a single protein. All the set of peptides collected were further screened to identify the peptides having highest antigenic propensity using the PVS server. Later the Surface accessibility Area of the selected peptides was calculated to eliminate the peptides having lowest SAA so as to increase the accuracy and effectively of the vaccine thus generated. The final list of selected peptides was designed in Argus lab and their internal energy values were calculated. The peptides having lowest energy can be selected as the targets for the development of protein based vaccines. The work can further be extended to synthetically produce the antigenic peptides and attenuate them so as to check for its capability of immunization on lab animals. Once the result shows positive in the lab animals its can be processed for the production of large scale vaccines for MERS so as to eradicate the disease.