Abstract
The virus was identified on the basis of host range, symptomatology, stability in crude extracts, modes of transmission, serological reaction, inclusion bodies and electron microscopy. The virus was purified from infected plants using the purified procedure. The UV-absorption spectrum had a maximum at 260 nm and a minimum at 245 nm. The ratios of A(max)/A(min) and A(260)/A(280) were 1.19 and 1.25 respectively.
The yield of purified pepper mild mottle virus (PMMoV) preparation was about 5mg/100g of infected tissue collected on the basis of an extinction coefficient 3.18 and polyclonal antibodies raised against PMMoV had a maximum titer of 1: 1024 from bleeding taken 12 days after the last injection. The validity of antiserum production was determined by tissue blot immuno binding assay (TBIA) and dot blot immuno binding assay (DBIA).