Abstract
The objective of this research was to develop and validate an analytical method for quantitative determination of fluoroquinolones using multivariate calibration technique in order to reduce chance errors. Simple and rapid chromatographic method for quantitative determination of four quinolone antibiotics, separately in pharmaceutical formulations and human serum, was developed and validated. The studied fluoroquinolones were pefloxacin, norfloxacin, ciprofloxacin and ofloxacin. These quinolones were analysed using a Nucleosil C-18 (10 mu m, 25 cm x 0.46 cm) column, a water acetonitrile (50:50, v/v) mobile phase of pH 2.9 adjusted with phosphoric acid, and propylparaben as an internal standard. The flow rate was 1.0 mL min(-1). The analyses were performed at the room temperature (24 +/- 2 degrees C) using a UV detector at the wavelengths 260, 265, 270, 275 and 280 nm. All fluoroquinolones were separated within 10 min. The calibration curves were linear (r >= 0.9999) over the concentration range 20-10000 ng mL(-1). Relative standard deviation (RSD) was < 1.56%, minimum recovery was 98.65% and maximum recovery equaled to 100.81%.