Abstract
Large variations were found in the effectiveness of five reversed-phased high-performance liquid chromatography columns used for the analysis of free and conjugated flavonoids. The best results were obtained with C
18 Nova-Pak, C
18 Symmetry and C
18 Genesis columns but substantial band broadening and peak asymmetry were observed when free flavonoids were chromatographed on ODS-Hypersil and LiChrospher RP-18 supports. The Symmetry and Genesis columns provided gradient elution separations of rutin, quercetin-3-glucoside, quercitrin, myricetin, luteolin, quercetin, apigenin, kaempferol and isorhamnetin. This procedure was used for the quantitative analysis of endogenous flavones and flavonols in acid hydrolysed extracts from onions and celery.