Abstract
Molecular markers enable the detection and classification of fungi isolated from their natural environments. To develop species-specific markers for detecting
Trichoderma koningiopsis
and
T. longibrachiatum
, the sequence-characterized amplified region technique, using 20 inter-simple sequence repeat-polymerase chain reaction primers, was performed. The two specific markers for amplifying a single unique band consistent with
T. koningiopsis
and
T. longibrachiatum
, which were absent with other
Trichoderma
strains, were successfully identified. These fragments had no meaningful sequence homology with known sequences available in the National Center for Biotechnology Information and TrichOKEY databases. Compared with traditional identification techniques, these markers can facilitate more rapid and less complicated studies of
Trichoderma
population dynamics and evaluate their establishment after release into agricultural environments.