Abstract
Anion sensing via small molecules as chemosensor carries unique significance in biological and environmental fields, where it offers several advantages over the traditional methods of ion sensing. In the current study, the receptor-spacerfluorophore based coumarin thiosemicarbazones were synthesized and investigated as chemosensor against different anions. The anion interaction with thiosemicarbazone based receptors (ligands) was observed and investigated with naked-eye and by using UV-Visible, fluorescence and (HNMR)-H-1 spectroscopic techniques. The deprotonation of NH protons upon binding with F was confirmed via (HNMR)-H-1 spectroscopic data. Binding constants and limit of detection was also calculated. Theoretical studies via DFT calculations were also carried to understand the ligand-anion interaction. The theoretical and experimental data was found to be in agreement, confirming the anion mediated NH proton abstraction as the mechanism that is responsible for anion sensing. Furthermore, the reason (and mechanism responsible) for anion selectivity exhibited by these chemosensors was also investigated computationally.