Abstract
In this study, we reconstructed for the first time a genome-scale metabolic model (GSMM) of
Ganoderma lucidum
strain CGMCC5.26, termed model
i
ZBM1060, containing 1060 genes, 1202 metabolites, and 1404 reactions. Important findings based on model
i
ZBM1060 and its predictions are as follows: (i) The extracellular polysaccharide (EPS) biosynthetic pathway was elucidated completely. (ii) A new fermentation strategy is proposed: addition of phenylalanine increased EPS production by 32.80% in simulations and by 38.00% in experiments. (iii) Eight genes for key enzymes were proposed for EPS overproduction. Model
i
ZBM1060 provides a useful platform for regulating EPS production in terms of system metabolic engineering for
G. lucidum
, as well as a guide for future metabolic pathway construction of other high value-added edible/ medicinal mushroom species.