Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Sanu Arora; Burkhard Steuernagel; Kumar Gaurav; Sutha Chandramohan; Yunming Long; Oadi Matny; Ryan Johnson; Jacob Enk; Sambasivam Periyannan; Narinder Singh; M Asyraf Md Hatta; Naveenkumar Athiyannan; Jitender Cheema; Guotai Yu; Ngonidzashe Kangara; Sreya Ghosh; Les J Szabo; Jesse Poland; Harbans Bariana; Jonathan D G Jones; Alison R Bentley; Mick Ayliffe; Eric Olson; Steven S Xu; Brian J Steffenson; Evans Lagudah; Brande B H Wulff

doi:10.1038/s41587-018-0007-9

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Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Journal article

Peer reviewed

Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Sanu Arora, Burkhard Steuernagel, Kumar Gaurav, Sutha Chandramohan, Yunming Long, Oadi Matny, Ryan Johnson, Jacob Enk, Sambasivam Periyannan, Narinder Singh, …

Nature biotechnology, Vol.37(2), pp.139-143

01/02/2019

DOI: https://doi.org/10.1038/s41587-018-0007-9

PMID: 30718880

Abstract

Chromosome Mapping

Cloning, Molecular

Crops, Agricultural - genetics

Disease Resistance - genetics

Genes, Plant

Genetic Association Studies

Genetic Variation

Genomics

Genotype

Models, Genetic

Phenotype

Phylogeny

Plant Diseases - genetics

Polymorphism, Single Nucleotide

Seedlings

Triticum - genetics

Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.

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Title: Resistance gene cloning from a wild crop relative by sequence capture and association genetics
Creators - without role: Sanu Arora - Norwich Research Park
Burkhard Steuernagel - Norwich Research Park
Kumar Gaurav - Norwich Research Park
Sutha Chandramohan - Agriculture and Food
Yunming Long - North Dakota State University
Oadi Matny - University of Minnesota
Ryan Johnson - University of Minnesota
Jacob Enk - Aastrom Biosciences
Sambasivam Periyannan - Agriculture and Food
Narinder Singh - Kansas State University
M Asyraf Md Hatta - Universiti Putra Malaysia
Naveenkumar Athiyannan - Agriculture and Food
Jitender Cheema - Norwich Research Park
Guotai Yu - Norwich Research Park
Ngonidzashe Kangara - Norwich Research Park
Sreya Ghosh - Norwich Research Park
Les J Szabo - Cereal Disease Laboratory
Jesse Poland - Kansas State University
Harbans Bariana - University of Sydney
Jonathan D G Jones - Sainsbury Laboratory
Alison R Bentley - National Institute of Agricultural Botany
Mick Ayliffe - Agriculture and Food
Eric Olson - Michigan State University
Steven S Xu - Edward T. Schafer Agricultural Research Center
Brian J Steffenson - University of Minnesota
Evans Lagudah - Agriculture and Food
Brande B H Wulff - Norwich Research Park
Publication Details: Nature biotechnology, Vol.37(2), pp.139-143
Grant note: BB/H019820/1 / Biotechnology and Biological Sciences Research Council BB/P021646/1 / Biotechnology and Biological Sciences Research Council BB/L009293/1 / Biotechnology and Biological Sciences Research Council BB/E006868/1 / Biotechnology and Biological Sciences Research Council
Identifiers: 9944477508331
Academic Unit: King Abdullah University of Science & Technology
Language: English
Resource Type: Journal article