Abstract
Objectives: To determine the relationship between resistin gene (RETN) C-180G variant and circulating resistin concentration in Saudi colon cancer patients.
Methods: This case-control study was conducted in the Biochemistry Department, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia from April 2009 to December 2009. The serum concentration had been measured with enzyme-linked immunosorbent assay in 60 colon cancer patients and in 60 controls matched in gender and age. The single nucleotide polymorphism (SNP) C-180G was genotyped using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) techniques.
Results: We observed a significantly higher serum resistin level in colon cancer group compared with control group (19.44 +/- 8.46 versus 5.45 +/- 2.73 ng/ml; p=0.0001), with significant (p=0.03) higher levels showed in women than in men in patients and controls. In patients, the heterozygous (CG) and homozygous (GG) genotype carriers showed higher (p=0.08) levels of serum resistin compared to CC homozygous. This difference was not observed (p=0.78) among SNP C-180G genotypes in control group.
Conclusion: Our result showed no association between the C-180G SNP and the serum resistin concentrations and suggests that the high resistin level in colon cancer patients may play an important role in colon cancer development.