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Robust Real-Time Reverse Transcription-PCR for Detection of Foot-and-Mouth Disease Virus Neutralizing Carryover Contamination
Journal article   Peer reviewed

Robust Real-Time Reverse Transcription-PCR for Detection of Foot-and-Mouth Disease Virus Neutralizing Carryover Contamination

Ji-Hyeon Hwang, Yong-Keol Shin, So-Yeon Park, Jeesoo Kim, Su-Mi Kim, Byounghan Kim, Jong-Hyeon Park, Jong-Soo Lee, Kwang-Nyeong Lee and Essam Hassan Ibrahim
Journal of clinical microbiology, Vol.54(1), pp.216-219
01/01/2016
DOI: https://doi.org/10.1128/JCM.01944-15
PMCID: PMC4702723
PMID: 26560537

Abstract

Animals Decontamination - methods DNA Restriction Enzymes - metabolism Equipment Contamination Foot-and-Mouth Disease - diagnosis Foot-and-Mouth Disease - epidemiology Foot-and-Mouth Disease Virus - isolation & purification Real-Time Polymerase Chain Reaction - methods Reverse Transcriptase Polymerase Chain Reaction - methods
During an outbreak of foot-and-mouth disease (FMD), real-time reverse transcription-PCR (rRT-PCR) is the most commonly used diagnostic method to detect viral RNA. However, while this assay is often conducted during the outbreak period, there is an inevitable risk of carryover contamination. This study shows that the carryover contamination can be prevented by the use of target-specific restriction endonuclease in that assay.

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