Abstract
The simple and sensitive UV-visible spectrophotometric analytical methods have been adopted for the determination and validation of ZnO nanostructures (nanorods (NRs), nanosphere (NSs), and nanonuts (NNTs)), concentration, which are used to control the bacterial growth of Escherichia coli. This method is based on processing conditions of nanostructures of ZnO using precursors zinc acetate dihydrate (Zn(AC(2))(2)center dot 2H(2)O), zinc nitrate hexahydrate (Zn(NO3)(2)center dot 6H(2)O), sodium hydroxide, hexamethylenetetramine (HMT), methanol, etc. The optical density (OD) of the resulting solution of ZnO nanostructures with E. coli bacteria were measured at 600 nm against the reagent blank, prepared under the same conditions. The use of statistical analysis for evaluation of the resulting solution (ZnO-NRs, ZnO-NSs, and ZnO-NNTs with E. coli) was optimized and validated by various operational parameters. Beer's law was followed (Concentration range from 0.25-2.0 mu g ml(-1)) with apparent molar absorptivity of 4.38 x 102 l mol(-1) cm(-1) for ZnO-NRs, 2.70 x 10(2) l mol(-1) cm(-1) for ZnO-NSs, and 3.10 x 102 l mol(-1) cm(-1) for ZnO-NNTs, respectively. The calibration curve shows linearity over the concentration range of 0.25-1.50 mu g ml-1 for ZnO-NRs and 0.25-2.0 mu g ml(-1) for ZnO-NSs and ZnO-NNTs. Detection limit (LOD) and quantitation limit (LOQ) were found to be 0.022: 0.068 mu g ml(-1) for ZnO-NRs, 0.028: 0.087 mu g ml(-1) for ZnO-NSs, and 0.044: 0.137 mu g ml(-1) for ZnO-NNTs analyzed by spectrophotometric method, respectively.