Abstract
Infertility has evolved into a worldwide reproductive health concern that is distinctively constituted within various socio-cultural contexts. Female infertility is a global health concern ailment in couples who have been unable to conceive for a year. Kisspeptin (KISS1) is a peptide hormone that is important in women's health difficulties such as infertility. Based on previous studies, single nucleotide polymorphisms (SNPs) in KISS1 gene can be a cause for infertility. The objective of this study is to explore into the Q36R SNP in the KISS1 gene in Saudi infertile women.
In this case-control study, 96 cases of female infertile and 96 control women was selected. Deoxyribonucleic acid (DNA) was extracted to perform polymerase chain reaction and followed by restriction fragment length polymorphism analysis. All DNA products were run on 2% agarose and obtained data was imported into excel to perform the statistical analysis using SPSS software between cases and controls.
The anthropometric analysis (p > 0.05), genotype (OR-3.60; 95 %CI:0.96–13.55; p = 0.04), allele (OR-2.49; 95 %CI: 0.86–7.21; p = 0.08) and different genetic models (AG + GG vs AA; OR-1.00; 95 %CI: 0.01–7.02; p = 0.005) showed negative association and fertility histories showed significant association (p < 0.0001). Anova analysis showed the positive association with weight (p = 0.01) and BMI (p = 0.003).
This study found no connection between the Q36R SNP in the KISS1 gene and female infertility in Saudi Arabia.