Abstract
The association between vitamin D and cancer has been studied with different focuses. As far as the beneficial effects concern, studies have shown the effects of vitamin D in inflammatory response and oxidative stress in relation with cancer regression. Indirect anti-cancer effects of vitamin D also due to its anti-inflammatory properties. Nuclear factor kappa B (NF-kappa B) is considered as a major mediator of inflammation and key transcription factor linking inflammation of cancer development. On the other hand, caspase protein also has an important role in apoptosis. Further, apoptosis and the BCL-2 family now inextricably linked to human cancer. Since the vitamin D-3 has proven to exert numerous tumor suppressive effects, the relationship of vitamin D-3 on the regulation of apoptosis and NF-kappa B is yet to be studied in detail. Hence, the present study aims at evaluating the efficacy of vitamin D-3 on NF-kappa B and Caspase 3 in the human colorectal carcinoma cell line (HCT116). Treatment of HCT cell line with different concentration of vitamin D-3 shown a dose-dependent effect on the expression of Caspase 3 not on NF-kappa B. This finding suggests that vitamin D does not have any effect on NF-kappa B, but have a positive effect on Caspase3 on HCT116, which might play a vital role in preventing the colon cancer. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.