Abstract
Helicobacter pylori causes gastritis, ulcer and gastric cancer. Bacterial infections may lead to epigenetics alteration via Sirtuin 2 in inflammatory and carcinogenesis process. In this case control study, expression of Sirt2 gene in gastric cancer with respect to H. pylori was investigated.
A total of 20 biopsies samples were obtained from patients with GI disturbances and gastric cancer. H. pylori was detected in staining, biochemical and species-specific PCR. After confirmation of H. pylori, total RNA was extracted for cDNA synthesis from gastric cancer biopsy of case and control samples. Real Time PCR was used to check the expression of Sirt2 gene.
Among the 20 biopsies, gastric ulcer (n=5), gastritis (n=2) and gastric cancer (n=12) were diagnosed with endoscopy and histopathology. H. pylori was confirmed in three samples of gastric cancer. On real time PCR, the expression of Sirt2 gene was increased by 2.6-fold; however non-significant (p> 0.05) correlation was observed between case and control samples. Findings of this study will help in H. pylori role in gastric cancer via Sirt2 gene expression.