Abstract
Iron nanocomposite adsorbent was prepared by green technology with 90% yield. The prepared iron nanocomposite adsorbent was used in solid-phase micromembrane tip extraction (SPMMTE) sample preparation technique. Analysis of atenolol and atorvastatin was performed in human plasma using SPMMTE and Q-TOF-UPLC-MS methods. New generation Acquity UPLC HSS penta fluoro phenyl (2.1 × 75 mm
2
; 1.8 µm) column was used with acetonitrile-0.1% formic acid in water (50:50 v/v) as mobile phase. The flow rate was 0.2 mL min
−1
with electrospray mass detection. The limits of detection were 0.2 and 0.4 ng active mass for atenolol and atorvastatin, while the limits of quantification were 1.0 and 2.0 ng active mass, respectively. The values of the retention times were 3.224 and 3.907 for atorvastatin and atenolol. The values of the separation and resolution factors were 1.31 and 1.71, respectively. The peaks were sharp with base lined separation within 4.2 min. The developed SPMMTE and Q-TOF-UPLC-MS methods were reproducible, fast, precise, robust, rugged, and economic for the analyses of atenolol and atorvastatin in human plasma. The reported methods can be applied for monitoring of the reported drugs at trace level.