Abstract
Tobaco mosaic virus (TMV) is a deadly plant virus with serious economic implications resulting in decrease crop-yield production. In recent years, environmentally friendly components for managing crop diseases have been developed as alternatives to chemical pesticides, including the use of agrowaste like pomegranate peels, Our studies were carried out to determine the chemical constituents of pomegranate peels and their sonicated nanoparticles. The present study is aimed to study the toxic effect of Tobaco mosaic virus (TMV) infections with either pomegranate peels (PPs) and their nanoparticles (PPNPs) extract treatments as antioxidant and antiviral agent by using the local lesion assay on Datura motel. The virus was obtained from infected tobacco plants. The pomegranate peels were extracted by phosphate buffer, pH7.2 and the homogenates were used itself or after developed based on the ultrasound then the transmission electron microscopy (TEM) analysis showed that nanoparticles ranged from 15 to 30 nm. Chemical composition compounds were determined; also, genetic diversity of plant samples was estimated using RAPD and. ISSR molecular markers. Our results showed that the antiviral extracts of PPs and. PPNPs used had significant effects in decreasing the number of local lesions formed by the challenge inoculation of TMV. The PPNPs extract was found to be the highest effective against TMV, which may be due to their chemical composition which contain high concentrations of monosaccharides, amino acids, phenolic and flavonoids compounds and antioxidant activity (DPPH). In addition, Results showed that tested extracts obtained from PPS or PPNP have no effect on DNA of Datura motel Plants, At the same time, the viral infection was inhibited, which made them safe as biological materials, Therefore, this study recommends the application of the agrowaste (PPS or PPNPs) as ecofriendly antiviral.