Abstract
Nearly a billion people, especially in underdeveloped nations, need safe drinking water. Indian studies suggest that most drinking water sources have high coliform levels, and quality assurance is required. This study was conducted in rural parts of South Kashmir in the Western Himalaya from February 2019 to January 2020. Standard river water sampling was done from upstream to downstream of the river. This study examined the detection, molecular identification, and chemical water quality of coliform-contaminated drinking water, which sums up river water pollution. Water quality varied significantly, indicating downstream contamination. Sangam (downstream) had the highest coliform count, showing 72.2600 cfu per litre in summer, while Kongwaton (upstream), near the Veshaw River, had no coliform count in winter. In summer, Sangam (downstream) had the highest water quality metrics (pH 6.847, Electrical conductivity (EC) 71.620 dS/m, Biological oxygen demand (BOD) 1.120 mg/L, and Chemical oxygen demand (COD) 24.637 mg/L) in all seasons. The lowest winter water quality metrics in Kongwaton were pH 8.947, EC 253.680 dS/m, BOD 4.963 mg/L, and COD 51.440 mg/L. Coliforms in water suggest faecal contamination. This study examines the water quality attributes of drinking water and associated factors to determine river pollution. Total DNA was collected and sequenced for 16 S rDNA and metagenomics. Universal primers were used to amplify the bacterial 16 S rRNA. Using BLAST, the amplified 16 S rRNA gene sequence was matched to the NCBI database. A metagenomic study revealed 27 species with different relative abundance. These species include Escherichia coli, E. fergusonii, E. albertii, Klebsiella grimontii, and Shigella dysenteriae. This study is thought to be the first to discriminate against E. fergusonii, E. albertii, K. grimontii, and S. dysenteriae from E. coli and to report on E. fergusonii and E. albertii, K. grimontii, and S. dysenteriae in the river Veshaw water sources in Kulgam, Western Himalaya.
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•Seasonal microbial shift, drinking water quality & molecular detection of bacterial species all help to improve water quality.•Natural and anthropogenic pollutants in this stream affect water quality spatially and seasonally.•E. fergusonii, E. albertii, Klebsiella grimontii, & Shigella dysenteriae can monitor river pollution.•This freshwater body needs supervision to safeguard aquatic life and humans.