Abstract
The synthesis of metal nanoparticles using algae has been unexplored, but it is a more biocompatible method than the other biological methods. Metal nanoparticle synthesis using algae extract shows rapid and non- toxic process which resulted to nano sizes having the greatest potential for biomedical applications. In this investigation, we studied the green synthesis of gold nanoparticles using the algae extract of Turbinaria conoides. Green synthesis of gold nanoparticles was preliminarily confirmed by color changing from yellow to dark pink in the reaction mixture, and the broad surface plasmon resonance band was centered at 520 to 525 nm which indicates polydispersed nanoparticles. Transmission electron microscopy and selected-area electron diffraction analysis show the morphology and crystalline structure of synthesized gold nanoparticles with the size range of 6 to 10 nm. The four strong diffraction peaks were observed by X-ray diffraction; it confirmed the crystalline nature of synthesized gold nanoparticles. The carboxylic, amine, and polyphenolic groups were associated with the algae-assisted synthesized gold nanoparticles which was confirmed using Fourier transform-infrared spectroscopy. This study eliminates the use of chemical substances as reducing and stabilizing agent. Because it has natural several constituents which are fucoidan and polyphenolic substances, it does a dual function as both reducing and stabilizing agent for nanoparticles. Thus, algae-mediated synthesis process of biomedically valuable gold nanoparticles is a one-spot, facile, convenient, large-scaled, and eco-friendly method.