Abstract
Preliminary aim of this research work focuses on testing the efficacy of silver nanoparticles synthesised using water soluble polysaccharides extracted from seaweeds against pathogenic fish bacteria. The emergence of various virulence pathogens during the culturing period leads to mass mortality and influence the loss of aquaculture production.
The intervention of nanotechnology in the field of aquaculture improves the growth and immunity of aquatic animals when the part of nanomaterials mixed with fish feed. Sometimes the green synthesis of silver nanoparticles prepared from the seaweed polysaccharide play a vital role in the disease controlling strategies in aquaculture farming. The nanoparticle formation and confirmation was charecterized by Ultra Visible Spectroscopy (UV–Vis), Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscope (SEM), Dynamic Light Scattering (DLS).
Biologically synthesized silver nanoparticles have been used as an alternative method for commercial antibiotics to control such pathogenic infection with 70–80% of survival rate. Polysaccharides of Caulerpa racemosa was used as a reducing agent in this present study. Particularly the phytochemicals are involved in the silver salt reduction process. Total polyphenol, total flavonoids, total antioxidant, hydroxyl radical scavenging assay and DPPH was found to be 12.32 mg/g, 18.44 mg/g, 38.12 mg/g equivalence, 82.2% and 49.05% respectively. Biologically synthesised AgNps capable of preventing the Pseudomonas aeruginosa infection in tilapia, it was proved by in vitro and in vivo antibacterial activities. Present study provided the ample achievement and these findings found to be effective in controlling the Pseudomonas infection in tilapia fishes.