Abstract
A series of semicarbazone, thiosemicarbazone, and pyrazole derivatives (3a-3m) were designed, synthesized, and assessed for monoamine oxidase inhibition using two isoforms.
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•A series of hydrazine-carboxamide, hydrazine-carbothioamide, and pyrazole analogues were synthesized.•Examined for monoamine oxidase inhibition using two isoforms, MAO-A and MAO-B.•Molecular dockingandin-silicoADMEevaluation.
A series of semicarbazone, thiosemicarbazone, thiazole, and oxazole derivatives were designed, synthesized, and examined for monoamine oxidase inhibition using two isoforms, i.e., MAO-A and MAO-B. Among all the analogues, 3c and 3j possessed substantial activity against MAO-A with IC50 values of 5.619 ± 1.04 µM and 0.5781 ± 0.1674 µM, respectively. Whereas 3d and 3j were active against monoamine oxidase B with the IC50 values of 9.952 ± 1.831 µM and 3.5 ± 0.7 µM, respectively. Other derivatives active against MAO-B were 3c and 3g with the IC50 values of 17.67 ± 5.6 µM and 37.18 ± 2.485 µM. Moreover, molecular docking studies were achieved for the most potent compound (3j) contrary to human MAO-A and MAO-B. Kinetic studies were also performed for the most potent analogue to evaluate its mode of interaction with MAO-A and MAO-B.