Abstract
In this paper, two simple, rapid and highly sensitive spectrofluorimetric methods were developed and validated for nystatin determination in its pure form and pharmaceutical dosage form (oral suspension). The first method was based on measuring the nystatin native fluorescence after dilution with isopropyl alcohol at 407 nm (excitation 303 nm). The fluoresence intensity was linearly dependant on the nystatin concentration within the specified range 50–500 ng ml−1. The second was based on micellar enhancement of nystatin fluorescence using sodium dodecyl sulphate (SDS). In the presence of 2% w/v SDS, an ~1.9‐fold enhancement could be achieved in the relative fluorescence intensity of nystatin. The linear range for the second method was 20–100 ng ml−1. The limits of quantification and detection were found to be 43.23 ng ml−1 and 14.27 ng ml−1 (Method I), 6.08 ng ml−1 and 2.0 ng ml−1 (Method II). According to percentage recoveries and relative standard deviations (RSDs) obtained, the proposed methods were precise (RSDs were less than 2%), reproducible, and accurate and could be successfully applied for quantitative estimation of nystatin in its dosage form. The statistical results of this method were compared with that of the reported method and showed excellent agreement with respect to accuracy and precision.
The fluorescence spectrum of nystatin (50 ng ml−1), where: (A) and (B) are excitation and emission spectra of aqueous native nystatin solution, respectively, and (C) and (D) are excitation and emission spectra of enhanced nystatin fluorescence in the presence of 2% w/v sodium dodecyl sulphate, respectively.