Abstract
A new HPLC method has been developed for the chromatographic separation and simultaneous quantitative determination of anti-oxidant compounds (vitamin C, gallic acid, rutin and ellagic acid) in pomegranate freeze dried juice samples. Separation was accomplished on a C-18 column (250 x 4.6mm, particle size 5 mu m, Merck, Germany) with a gradient elution and recorded at 254 nm. The flow rate was kept as 1.0 mL(-1). All calibration curves showed good linear regression (r(2) >= 0.9925) within test ranges. Low LOD & LOQ values proved the sensitivity of the method. The proposed method was validated according to ICH guide lines for accuracy (97.2-102.5%), precision (0.12-1.87% RSD), and robustness (0.26-2.30% RSD). The results demonstrated that the proposed method is reproducible, accurate, economic and suitable for the quality control of different commercial products which contain pomegranate as an ingredient.