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Separation of cells involved in the mixed leukocyte culture by velocity sedimentation
Journal article   Peer reviewed

Separation of cells involved in the mixed leukocyte culture by velocity sedimentation

A.H Ragab and D.H Cowan
Cellular immunology, Vol.7(2), pp.336-340
01/01/1973

Abstract

Cells from the peripheral blood of two unrelated individuals were separated by velocity sedimentation, and fractions containing “pure” granulocytes and lymphocytes were prepared. One-way mixed leukocyte cultures (MLC's) were performed using these fractions. It was noted that the lymphocyte-versus-lymphocyte fractions gave a good response in the MLC and addition of granulocytes inhibited the response.

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